Code | CSB-EP621686HU |
MSDS | |
Size | US$306 |
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The cDNA fragment encoding 21-305aa of Human Deoxyribonuclease gamma (DNASE1L3) was fused with an N-terminal GST-tag and then expressed in E.coli. The product obtained is the recombinant full-length of mature human DNASE1L3 protein. Its purity was determined by using SDS-PAGE and reached up to 90%. Under the reducing conditions, the gel presented a molecular mass band of about 62 kDa. The slightly higher result was attributed to glycosylation. It was also validated by the LC-MS/MS analysis. In-stock DNASE1L3 proteins are offered now. This recombinant DNASE1L3 protein may find uses in the specific antibody generation or the studies of cell biology.
DNASE1L3 is a secreted DNASE1-like nuclease, which can digest DNA in chromatin. The deletion of DNASE1L3 can cause anti-DNA responses and autoimmunity in humans and mice. Lee Serpas et al. proved that DNASE1L3 plays a role in circulating plasma DNA homeostasis by enhancing fragmentation and affecting terminal motif frequencies. These results support the unique role of DNASE1L3 as a regulator of physical form and cell-free DNA availability, which may be significant for the DNASE1L3's mechanism of preventing autoimmunity.
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I am confirming if CSB-EP621686HU has been tested for activity?
These proteins are very popular, among which CSB-EP621686HU protein has received feedback from customers of different pharmaceutical companies, and the effect is good, and all of them have been renewed for many times.
However, because the customers of pharmaceutical companies have not fed back detailed corresponding data and plans, we are temporarily unable to provide customers with reference.
According to the feedback from customers of pharmaceutical companies, CSB-EP621686HU should be active, but since we have not tested the activity and sold it as an active protein yet, there is no guarantee that it will be 100% active. If researchers are interested in the activity of this protein, they need to determine its biological activity according to their own practical experience.